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| Registros recuperados : 37 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
15/02/2016 |
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Data da última atualização: |
15/02/2016 |
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Autoria: |
SANTOS, E. D.; SILVA FILHO, J. R.; MACHADO, T. P.; DAU, S. L; RODRIGUEZ, R.; MOTTA, A C. da. |
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Afiliação: |
EZEQUIEL D. SANTOS, FAMV; JOSÉ R. SILVA FILHO, Universidade de Passo Fundo; TANISE P. MACHADO, FAMV; STEFANO L. DAU; RUBENS RODRIGUEZ, IPPF; ADRIANA C. da MOTTA, FAMV. |
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Título: |
Oral fibrosarcoma in jararaca (Bothrops pubescens): anatomopathological and immunohistochemical aspects. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira v. 35, n.7, p. 664-670, julho 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
A 4-year-old female captive-bred snake of the genus Bothrops showed swelling on the left side of the oral cavity, suggesting the development of neoplasia. The mass was removed surgically and sent for pathological examination. Two months later a new increase in volume in the same site was observed, suggesting recurrence. The lesion was completely removed and sent for pathological analysis. Histologically, the two-samples consisted of a mass with highly-cell density composed of spindle-shaped anaplastic cells arranged in interwoven bundles, distributed throughout the tissue extension and, occasionally, polygonal cells arranged in irregular fascicles. The Masson trichrome staining showed modest amount of collagen supporting the neoplastic cells. PAS-positive content was not observed in the cytoplasm of neoplastic cells. Histological and histochemical findings indicated that it was a spindle cell neoplasm, but the classification was not possible. Immunohistochemistry was requested and performed using the streptavidin-biotin-peroxidase method. The markers used were anti-vimentin, anti-PCNA, anti-EMA, anti-melan A and anti-melanosome, antidesmin, anti-actin, anti-CD68 and anti- S100protein. The neoplastic cells were immunoreactive for vimentin and PCNA and negative for the other antibodies. The morphology characterization, histochemical and immunohistochemical analysis of neoplastic cells allowed the definitive diagnosis of oral fibrosarcoma. |
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Palavras-Chave: |
Fibrossarcoma; Histochemical; Imuno-histoquímica; Neoplasma fusocelular; PCNA; Serpente; Vimentina. |
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Thesagro: |
Histoquímica. |
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Thesaurus NAL: |
Bothrops; Fibrosarcoma; Immunohistochemistry; Proliferating cell nuclear antigen; Snakes; Vimentin. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/139060/1/Oral-fibrosarcoma.pdf
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Marc: |
LEADER 02436naa a2200349 a 4500
001 2037054
005 2016-02-15
008 2015 bl uuuu u00u1 u #d
100 1 $aSANTOS, E. D.
245 $aOral fibrosarcoma in jararaca (Bothrops pubescens)$banatomopathological and immunohistochemical aspects.
260 $c2015
520 $aA 4-year-old female captive-bred snake of the genus Bothrops showed swelling on the left side of the oral cavity, suggesting the development of neoplasia. The mass was removed surgically and sent for pathological examination. Two months later a new increase in volume in the same site was observed, suggesting recurrence. The lesion was completely removed and sent for pathological analysis. Histologically, the two-samples consisted of a mass with highly-cell density composed of spindle-shaped anaplastic cells arranged in interwoven bundles, distributed throughout the tissue extension and, occasionally, polygonal cells arranged in irregular fascicles. The Masson trichrome staining showed modest amount of collagen supporting the neoplastic cells. PAS-positive content was not observed in the cytoplasm of neoplastic cells. Histological and histochemical findings indicated that it was a spindle cell neoplasm, but the classification was not possible. Immunohistochemistry was requested and performed using the streptavidin-biotin-peroxidase method. The markers used were anti-vimentin, anti-PCNA, anti-EMA, anti-melan A and anti-melanosome, antidesmin, anti-actin, anti-CD68 and anti- S100protein. The neoplastic cells were immunoreactive for vimentin and PCNA and negative for the other antibodies. The morphology characterization, histochemical and immunohistochemical analysis of neoplastic cells allowed the definitive diagnosis of oral fibrosarcoma.
650 $aBothrops
650 $aFibrosarcoma
650 $aImmunohistochemistry
650 $aProliferating cell nuclear antigen
650 $aSnakes
650 $aVimentin
650 $aHistoquímica
653 $aFibrossarcoma
653 $aHistochemical
653 $aImuno-histoquímica
653 $aNeoplasma fusocelular
653 $aPCNA
653 $aSerpente
653 $aVimentina
700 1 $aSILVA FILHO, J. R.
700 1 $aMACHADO, T. P.
700 1 $aDAU, S. L
700 1 $aRODRIGUEZ, R.
700 1 $aMOTTA, A C. da
773 $tPesquisa Veterinária Brasileira$gv. 35, n.7, p. 664-670, julho 2015.
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Embrapa Unidades Centrais (AI-SEDE) |
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